Journal: Molecular & Cellular Proteomics : MCP

Article Title: Integrative Proteogenomics for Differential Expression and Splicing Variation in a DM1 Mouse Model

doi: 10.1016/j.mcpro.2023.100683

Figure Lengend Snippet: Comparison of instances of differential alternative splicing at transcriptional and protein level for Atp2a1 and Bin1 genes. Panels on the left show LeafCutter AS intron clusters with relative exon usage in the DM1 and WT control groups. Panels in the middle show qRT-PCR results with the splicing-specific probes (see ) covering the corresponding exon inclusion ( gray ) and exon exclusion ( blue ). Panels on the right show the corresponding relative abundances of peptides specific to exon inclusion or exclusion in the same sample groups. See also supplemental Fig. S4 for a UCSC genome browser-based display of these LeafCutter AS intron clusters. AS, alternative splicing; DM1, myotonic dystrophy, type 1.

Article Snippet: Primary antibodies were obtained from Cell Signaling—Rb anti-BIN1 mAB (#51844) and Rb anti-SERCA1 mAb (#12293).

Techniques: Comparison, Alternative Splicing, Control, Quantitative RT-PCR

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Integrative Proteogenomics for Differential Expression and Splicing Variation in a DM1 Mouse Model

doi: 10.1016/j.mcpro.2023.100683

Figure Lengend Snippet: Overview of select genes of known importance to DM1, and summary of their performance in our work, summarized in columns (3) to (6)

Article Snippet: Primary antibodies were obtained from Cell Signaling—Rb anti-BIN1 mAB (#51844) and Rb anti-SERCA1 mAb (#12293).

Techniques: